Interactive IVF lab education

Semen Analysis Reference Explorer

Compare four common report values with the World Health Organization lower reference values without turning them into a score or diagnosis.

These values are reference points, not pass or fail lines. The WHO lower reference values describe the fifth-centile positions in a reference population of men whose partners conceived naturally within 12 months. A value on either side does not, by itself, confirm fertility or infertility.

Four common measurements

Read each number on its own terms

A complete semen analysis contains several measurements. This explorer focuses on four values patients often see. Enter a number from a report or move the slider. The response shows position only. It does not assign a grade or explain a cause.

Semen volume

WHO lower reference value1.4 mLSixth edition, 2021

Entered value: 1.4 mL

What it measures

The amount of semen collected in the sample container.

What it cannot tell you

It does not measure sperm concentration or establish fertility by itself.

Sperm concentration

WHO lower reference value16 million/mLSixth edition, 2021

Entered value: 16 million/mL

What it measures

The estimated number of sperm in one milliliter of semen.

What it cannot tell you

It does not state the total number in the ejaculate or establish fertility by itself.

Progressive motility

WHO lower reference value30%Sixth edition, 2021

Entered value: 30%

What it measures

The percentage of assessed sperm moving forward through the sample.

What it cannot tell you

It does not assess sperm DNA or predict one person’s chance of pregnancy.

Morphology, normal forms

WHO lower reference value4%Sixth edition, 2021

Entered value: 4%

What it measures

The percentage of assessed sperm that meet the laboratory’s defined shape criteria.

What it cannot tell you

It does not test sperm DNA or establish fertility by itself.

How to read the slider: The vertical marker is the WHO lower reference value. The response will tell you whether the number entered is below, at or above that reference point. This comparison does not determine fertility on its own.

The complete report still matters

One sample is one point in time

AUA/ASRM guidance explains that semen parameters are variable biological measurements and may change substantially from one ejaculate to another. The complete report, collection details, medical history and partner factors belong with the numbers.

This explorer does not assess total sperm number, total motility, vitality, pH, DNA fragmentation, hormones or the clinical history that may change how a care team reads the report.

Questions for the care team
  1. Which reference system and laboratory method were used for this report?
  2. How should these values be read alongside the other measurements and the collection details?
  3. Does the full history make repeat testing or evaluation by a male reproductive specialist relevant?
Male fertility, beyond the report

How hormone testing fits alongside semen analysis

In Video 2, I explain the male hormone axis, the tests that may add information to a semen analysis, and what those results can and cannot show.

More patient tools are coming

Watch this space

More plain-language explainers and interactive patient tools will be added here in the future.

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